Tagnaout, I. and Zerkani, H. and Mahjoubi, M. and Bourakhouadar, M. and Alistiqsa, F. and Bouzoubaa, A. and Zair, T. (2016) Phytochemical study, antibacterial and antioxidant activities of extracts of Capparis spinosa L. International Journal of Pharmacognosy and Phytochemical Research, 8 (12). pp. 1993-2005.

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Abstract

The Capparis spinosa L. is a species which has a great importance in the field of traditional medicine in both arid and semi-arid regions whose nutritional and medicinal value remains ambiguous in Morocco. It is in this context that our work is aiming at the recovery of this species through a phytochemical study and an evaluation of antibacterial and antioxidant activities of flower buds of Capparis spinosa L. spontaneous collected and sold by Nour cooperative within the region of Zerhoun, Morocco. We achieved the solid-liquid extraction by two different methods in order to optimize the extraction yields of polyphenols; maceration and soxhlet by using two mixtures of solvents: methanol / water and acetone/water at 70. The best yields are obtained with methanol / water mixture; 46 for soxhlet against 31 for maceration. The quantitative analysis of phenolic is being performed by colorimetry, extracts showed that the soxhlet method is more effective than the maceration. The qualitative analysis performed by HPLC / UV-ESI-MS also reported the existence of the same flavonoid glycosides in both hydromethanolic extracts in which the most important are the derivatives of kaempferol, quercetin and isorhamnetin. The antioxidant activity is determined by the DPPH test, showed that the aqueous-methanolic extracts obtained by soxhlet and maceration expressed some approximate antioxidant powers with IC50 of respectively 0.73± 0.01 and 0.84± 0.01 mg / ml. However, the antibacterial activity evaluated against pathogenic strains such as Staphylococcus aureus, Escherichia coli, Klebsilla pneumoniae, revealed the ineffectiveness of various extracts against the most tested strains. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.

Item Type: Article
Uncontrolled Keywords: acetone; anthocyanin; antiinfective agent; antioxidant; ascorbic acid; butylated hydroxyanisole; Capparis spinosa extract; coumarin; ellagic acid; flavonoid; gallic acid; isorhamnetin; kaempferol; leucoanthocyanin; methanol; phytosterol; plant extract; plant glycoside; polyphenol; quercetin; tannin derivative; triterpene; unclassified drug; water, antibacterial activity; antioxidant activity; Article; calibration; chemical procedures; colorimetry; concentration response; controlled study; DPPH radical scavenging assay; drug screening; electrospray mass spectrometry; Escherichia coli; extraction; flower; flower bud; high performance liquid chromatography; IC50; Klebsiella pneumoniae; maceration; Morocco; nonhuman; phytochemistry; process optimization; qualitative analysis; quantitative analysis; solid liquid extraction; Soxhlet extraction; Staphylococcus aureus
Subjects: Pharmacology, Toxicology and Pharmaceutics
Divisions: SCIENTIFIC PRODUCTION > Pharmacology, Toxicology and Pharmaceutics
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:49
URI: http://eprints.umi.ac.ma/id/eprint/4394

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