Maroui, I. and Barguigua, A. and Aboulkacem, A. and Ouarrak, K. and Sbiti, M. and Louzi, H. and Timinouni, M. and Belhaj, A. (2016) First report of VIM-2 metallo-β-lactamases producing Pseudomonas aeruginosa isolates in Morocco. Journal of Infection and Chemotherapy, 22 (3). pp. 127-132.

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Abstract

The emergence and the rapid spread of Pseudomonas aeruginosa carrying carbapenemases represent a serious threat to public health due to their delicate therapy. This work was performed to establish the resistance profile and to detect carbapenemases producing in 123 P. aeruginosa isolates. Among these 55 are environmental isolates and 68 are from the two major hospitals of Meknes-Tafilalet region in Morocco. All strains were tested against 14 antipseudomonal drugs by disc diffusion method. On carbapenem resistant strains minimum inhibitory concentrations of imipenem were determined by the E-test method. The modified Hodge test and EDTA tests were used for the detection of carbapenemases and metallo-β-lactamases (MBLs), respectively. PCR and DNA sequencing were conducted to detect carbapenemase-encoding genes and the enzyme types.12 of isolates was susceptible to all antibiotics tested and Carbapenem resistance was observed in 33 P. aeruginosa isolates, 33.3 of them were multi-drug resistant. Among carbapenem resistant strains only two (6.1) were positive for carbapenemases and also for MBLs. In addition to their resistance to almost all β-lactams tested, the MBLs producing strains were resistant to aminoglycosides. Molecular biology techniques confirmed the phenotypic results obtained for the two strains carbapenemase producers and demonstrated that each one of them carried blaVIM-2. The present study reports the first isolation of blaVIM genes in clinical isolates of P. aeruginosa in Morocco. Such isolates represent a serious emerging threat requiring strict hygiene measures to better control their spread. © 2015 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases.

Item Type: Article
Uncontrolled Keywords: bacterial DNA; antiinfective agent; beta lactamase; VIM-2 beta-lactamase, antibiotic sensitivity; antimicrobial activity; Article; bacterial strain; bacterium colony; bacterium isolation; controlled study; disk diffusion; DNA sequence; epsilometer test; inhibition zone; metallo beta lactamases producing Pseudomonas aeruginosa; minimum inhibitory concentration; Morocco; nonhuman; Pseudomonas aeruginosa; adult; antibiotic resistance; drug effects; enzymology; female; human; male; microbial sensitivity test; microbiology; middle aged; Pseudomonas aeruginosa; Pseudomonas infection; young adult, Adult; Anti-Bacterial Agents; beta-Lactamases; Drug Resistance, Bacterial; Environmental Microbiology; Female; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Morocco; Pseudomonas aeruginosa; Pseudomonas Infections; Young Adult
Subjects: Medicine
Divisions: SCIENTIFIC PRODUCTION > Medicine
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:49
URI: http://eprints.umi.ac.ma/id/eprint/4316

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