Yekkour, A. and Meklat, A. and Bijani, C. and Toumatia, O. and Errakhi, R. and Lebrihi, A. and Mathieu, F. and Zitouni, A. and Sabaou, N. (2015) A novel hydroxamic acid-containing antibiotic produced by a Saharan soil-living Streptomyces strain. Letters in Applied Microbiology, 60 (6). pp. 589-596.

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During screening for potentially antimicrobial actinobacteria, a highly antagonistic strain, designated WAB9, was isolated from a Saharan soil of Algeria. A polyphasic approach characterized the strain taxonomically as a member of the genus Streptomyces. The strain WAB9 exhibited a broad spectrum of antimicrobial activity toward various multidrug-resistant micro-organisms. A PCR-based assay of genomic potential for producing bioactive metabolites revealed the presence of PKS-II gene. After 6 days of strain fermentation, one bioactive compound was extracted from the remaining aqueous phase and then purified by HPLC. The chemical structure of the compound was determined by spectroscopic (UV-visible, and 1H and 13C NMR) and spectrometric analysis. The compound was identified to be 2-amino-N-(2-amino-3-phenylpropanoyl)-N-hydroxy-3-phenylpropanamide, a novel hydroxamic acid-containing molecule. The pure molecule showed appreciable minimum inhibitory concentration values against a selection of drug-resistant bacteria, filamentous fungi and yeasts. Significance and Impact of the Study: This study presents the isolation of a Streptomyces strain, named WAB9, from a Saharan soil in Algeria. This strain was found to produce a new hydroxamic acid-containing molecule with interesting antimicrobial activities towards various multidrug-resistant micro-organisms. Although hydroxamic acid-containing molecules are known to exhibit low toxicities in general, only real evaluations of the toxicity levels could decide on the applications for which this new molecule is potentially most appropriate. Thus, this article provides a new framework of research. © 2015 The Society for Applied Microbiology.

Item Type: Article
Uncontrolled Keywords: 2 amino n (2 amino 3 phenylpropanoyl) n hydroxy 3 phenylpropanamide; antibiotic agent; hydroxamic acid derivative; unclassified drug; antiinfective agent; hydroxamic acid; soil, antibiotics; carboxylic acid; genome; microbial community; molecular analysis; soil microorganism; toxicity, Algeria; antimicrobial activity; Article; bacterial gene; bacterial genome; bacterial strain; bacterium isolation; carbon nuclear magnetic resonance; drug isolation; drug purification; drug screening; fermentation; genomics; high performance liquid chromatography; metabolite; minimum bactericidal concentration; multidrug resistance; nonhuman; PKS II gene; polymerase chain reaction; proton nuclear magnetic resonance; soil microflora; Streptomyces; structure activity relation; ultraviolet spectroscopy; bacterium; biosynthesis; chemistry; drug effects; genetics; isolation and purification; metabolism; microbial sensitivity test; microbiology; soil; Streptomyces; yeast, Algeria; Sahara, Actinobacteria; Fungi; Streptomyces, Algeria; Anti-Bacterial Agents; Bacteria; Chromatography, High Pressure Liquid; Drug Resistance, Multiple, Bacterial; Drug Resistance, Multiple, Fungal; Hydroxamic Acids; Microbial Sensitivity Tests; Soil; Soil Microbiology; Streptomyces; Yeasts
Subjects: Immunology and Microbiology
Divisions: SCIENTIFIC PRODUCTION > Immunology and Microbiology
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:48

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