Belhaj, A. and Desnoues, N. and Elmerich, C. (2002) Alkane biodegradation in Pseudomonas aeruginosa strains isolated from a polluted zone: Identification of alkB and alkB-related genes. Research in Microbiology, 153 (6). pp. 339-344.

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Pseudomonas aeruginosa strains that grow on crude oil as the sole source of carbon and energy were isolated from an environment in Morocco polluted by petroleum refinery effluents. The twenty isolates grew on saturated alkanes from C12 to C22. Three of the isolates were also able to grow on low molecular weight C6 to C10 n-alkanes, but the other 17 strains were not. The strains were tested for alkB and alkB-related genes encoding alkane-1-monooxygenase (alkane hydroxylase). Oligonucleotide primers specific for the alkB gene of strain P. putida (GPo1) and for the alkB1 and alkB2 genes of P. aeruginosa strain PAO1 allowed amplification from the P. aeruginosa isolates of fragments similar to alkB1 and alkB2 genes of strain PAO1. Only 3 strains carried an alkB gene very similar to that of strain GPo1, and these strains were the same ones that could utilise C6 to C10 n-alkanes. © 2002 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.

Item Type: Article
Uncontrolled Keywords: alkane 1 monooxygenase; alkane derivative; bacterial protein; carbon; oligonucleotide; petroleum; protein alkB; unclassified drug, article; bacterial gene; bacterial genetics; bacterial growth; bacterium isolation; carbon source; energy resource; gene amplification; gene identification; gene sequence; genetic code; microbial degradation; molecular weight; nonhuman; pollution; priority journal; Pseudomonas aeruginosa; Pseudomonas putida; sequence homology; strain difference, Alkanes; Amino Acid Sequence; Bacterial Proteins; Biodegradation, Environmental; DNA Damage; DNA Primers; Escherichia coli Proteins; Mixed Function Oxygenases; Molecular Sequence Data; Petroleum; Polymerase Chain Reaction; Pseudomonas aeruginosa, Pseudomonas; Pseudomonas aeruginosa; Pseudomonas putida
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: SCIENTIFIC PRODUCTION > Biochemistry, Genetics and Molecular Biology
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:44

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