Meklat, A. and Bouras, N. and Zitouni, A. and Mathieu, F. and Lebrihi, A. and Schumann, P. and Spröer, C. and Klenk, H.-P. and Sabaou, N. (2013) Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 103 (4). pp. 771-776.

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Abstract

A novel halophilic actinomycete, strain H32T, was isolated from a Saharan soil sample collected in El-Oued province, south Algeria. The isolate was characterized by means of polyphasic taxonomy. Optimal growth was determined to occur at 28-32 C, pH 6.0-7.0 and in the presence of 15-25 (w/v) NaCl. The strain was observed to produce abundant aerial mycelium, which formed long chains of rod-shaped spores at maturity, and fragmented substrate mycelium. The cell wall was determined to contain meso-diaminopimelic acid and the characteristic whole-cell sugars were arabinose and galactose. The predominant menaquinones were found to be MK-10(H4) and MK-9(H4). The predominant cellular fatty acids were determined to be anteiso C17:0, iso-C15:0 and iso-C16:0. The diagnostic phospholipid detected was phosphatidylcholine. Phylogenetic analyses based on the 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Actinopolyspora. The 16S rRNA gene sequence similarity indicated that strain H32T was most closely related to 'Actinopolyspora algeriensis' DSM 45476T (98.8 ) and Actinopolyspora halophila DSM 43834T (98.5 ). Furthermore, the result of DNA-DNA hybridization between strain H32T and the type strains 'A. Algeriensis' DSM 45476T, A. halophila DSM 43834T and Actinopolyspora mortivallis DSM 44261T demonstrated that this isolate represents a different genomic species in the genus Actinopolyspora. Moreover, the physiological and biochemical data allowed the differentiation of strain H32T from its closest phylogenetic neighbours. Therefore, it is proposed that strain H32T represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora saharensis sp. nov. is proposed. The type strain is H32T (=DSM 45459T=CCUG 62966T). © 2012 Springer Science+Business Media Dordrecht.

Item Type: Article
Uncontrolled Keywords: arabinose; diaminopimelic acid; galactose; menaquinone; phosphatidylcholine; RNA 16S; sodium chloride, Actinopolyspora algeriensis; actinopolyspora halophila; Actinopolyspora mortivallis; Actinopolyspora saharensis; Algeria; article; bacterial growth; bacterial strain; cell wall; chemotaxonomy; controlled study; gene sequence; genotype; halophilic bacterium; isolation and purification; mycelium; nonhuman; nucleotide sequence; pH; phenotype; phylogeny; priority journal; soil analysis; species differentiation; temperature, Actinomycetales; Algeria; Bacterial Typing Techniques; Carbohydrates; Cell Wall; Cluster Analysis; Cytosol; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Hydrogen-Ion Concentration; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sodium Chloride; Soil Microbiology; Temperature; Vitamin K 2, Actinopolyspora; Actinopolyspora halophila; Actinopolyspora mortivallis; Mycelium (genus)
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: SCIENTIFIC PRODUCTION > Biochemistry, Genetics and Molecular Biology
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:44
URI: http://eprints.umi.ac.ma/id/eprint/1570

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