Meklat, A. and Bouras, N. and Zitouni, A. and Mathieu, F. and Lebrihi, A. and Schumann, P. and Spröer, C. and Klenk, H.-P. and Sabaou, N. (2013) Actinopolyspora righensis sp. nov., a novel halophilic actinomycete isolated from Saharan soil in Algeria. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 104 (3). pp. 301-307.

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Abstract

A novel halophilic actinomycete strain, H23T, was isolated from a Saharan soil sample collected in Djamâa (Oued Righ region), El-Oued province, South Algeria. Strain H23T was identified as a member of the genus Actinopolyspora by a polyphasic approach. Phylogenetic analysis showed that strain H23T had 16S rRNA gene sequence similarities ranging from 97.8 (Actinopolyspora xinjiangensis TRM 40136T) to 94.8 (Actinopolyspora mortivallis DSM 44261T). The strain grew optimally at pH 6.0-7.0, 28-32 C and in the presence of 15-25 (w/v) NaCl. The substrate mycelium was well developed and fragmented with age. The aerial mycelium produced long, straight or flexuous spore chains with non-motile, smooth-surfaced and rod-shaped spores. Strain H23T had MK-10 (H 4) and MK-9 (H4) as the predominant menaquinones. The whole micro-organism hydrolysates mainly consisted of meso-diaminopimelic acid, galactose and arabinose. The diagnostic phospholipid detected was phosphatidylcholine. The major cellular fatty acids were anteiso-C 17:0 (37.4 ), iso-C17:0 (14.8 ), iso-C15:0 (14.2 ), and iso-C16:0 (13.9 ). The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora righensis sp. nov. is proposed, with the type strain H23T (=DSM 45501T = CCUG 63368T = MTCC 11562T). © 2013 Springer Science+Business Media Dordrecht.

Item Type: Article
Uncontrolled Keywords: arabinose; chloramphenicol; diaminopimelic acid; erythromycin; fatty acid; galactose; kanamycin; lysozyme; menaquinone; penicillin derivative; phosphatidylcholine; RNA 16S; sodium chloride; streptomycin, Actinobacteria; Actinopolyspora mortivallis; Actinopolyspora righensis; Actinopolyspora xinjiangensis; Algeria; antibiotic resistance; antibiotic sensitivity; article; bacterial growth; bacterial spore; bacterial strain; bacterium identification; bacterium isolation; fatty acid analysis; genotype phenotype correlation; genus; halophilic bacterium; mycelium; nonhuman; nucleotide sequence; pH; phylogeny; priority journal; sequence homology; soil analysis; soil microflora; strain identification; temperature, Actinopolyspora; Actinopolyspora mortivallis; Mycelium (genus), Actinomycetales; Africa, Northern; Algeria; Bacterial Typing Techniques; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Hydrogen-Ion Concentration; Microscopy, Electron; Molecular Sequence Data; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sodium Chloride; Soil Microbiology; Temperature
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: SCIENTIFIC PRODUCTION > Biochemistry, Genetics and Molecular Biology
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:44
URI: http://eprints.umi.ac.ma/id/eprint/1568

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