Hassani, N.E.A.E. and Baraket, A. and Neto, E.T.T. and Lee, M. and Salvador, J.-P. and Marco, M.-P. and Bausells, J. and Bari, N.E. and Bouchikhi, B. and Elaissari, A. and Errachid, A. and Zine, N. (2017) Novel strategy for sulfapyridine detection using a fully integrated electrochemical Bio-MEMS: Application to honey analysis. Biosensors and Bioelectronics, 93. pp. 282-288.

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Abstract

Sulfapyridine (SPy) is a sulfonamide antibiotic largely employed as veterinary drugs for prophylactic and therapeutic purposes. Therefore, its spread in the food products has to be restricted. Herein, we report the synthesis and characterization of a novel electrochemical biosensor based on gold microelectrodes modified with a new structure of magnetic nanoparticles (MNPs) coated with poly(pyrrole-co-pyrrole-2-carboxylic acid) (Py/Py-COOH) for high efficient detection of SPy. This analyte was quantified through a competitive detection procedure with 5-4-(amino)phenylsulfonamide-5-oxopentanoic acid-BSA (SA2-BSA) antigens toward polyclonal antibody (Ab-155). Initially, gold working electrodes (WEs) of integrated biomicro electro-mechanical system (BioMEMS) were functionalized by Ppy-COOH/MNPs, using a chronoamperometric (CA) electrodeposition. Afterward, SA2-BSA was covalently bonded to Py/Py-COOH/MNP modified gold WEs through amide bonding. The competitive detection of the analyte was made by a mixture of a fixed concentration of Ab-155 and decreasing concentrations of SPy from 50 µg L−1 to 2 ng L−1. Atomic Force Microscopy characterization was performed in order to ensure Ppy-COOH/MNPs electrodeposition on the microelectrode surfaces. Electrochemical measurements of SPy detection were carried out using electrochemical impedance spectroscopy (EIS). This biosensor was found to be highly sensitive and specific for SPy, with a limit of detection of 0.4 ng L−1. This technique was exploited to detect SPy in honey samples by using the standard addition method. The measurements were highly reproducible for detection and interferences namely, sulfadiazine (SDz), sulfathiazole (STz) and sulfamerazine (SMz). Taking these advantages of sensitivity, specificity, and low cost, our system provides a new horizon for development of advanced immunoassays in industrial food control. © 2016

Item Type: Article
Uncontrolled Keywords: Aromatic compounds; Atomic force microscopy; BioMEMS; Biosensors; Electrochemical impedance spectroscopy; Electrodeposition; Electrodes; Food products; Food supply; Gold; Gold coatings; Microelectrodes; Nanomagnetics; Nanoparticles; Sulfur compounds; Synthesis (chemical), Electrochemical measurements; Electromechanical systems; Food control; Magnetic nanoparti cles (MNPs); Magnetic nanoparticles; Pyrrole-2-carboxylic acids; Sulfapyridine; Synthesis and characterizations, Chemical detection, 5 4 (amino)phenylsulfonamide 5 oxopentanoic acid; amide; copolymer; magnetic nanoparticle; poly(pyrrole co pyrrole 2 carboxylic acid); polyclonal antibody; sulfadiazine; sulfamerazine; sulfapyridine; sulfathiazole; unclassified drug; gold; metal nanoparticle; sulfapyridine, Article; atomic force microscopy; biosensor; chemical binding; chemical structure; concentration (parameters); electrochemical analysis; electrochemical biomicro electro mechanical system; electrochemical impedance spectroscopy; food analysis; food control; honey; immunoassay; microelectrode; sensitivity and specificity; synthesis; analysis; chemistry; genetic procedures; isolation and purification; procedures, Biosensing Techniques; Dielectric Spectroscopy; Food Analysis; Gold; Honey; Metal Nanoparticles; Microscopy, Atomic Force; Sulfapyridine
Subjects: Biochemistry, Genetics and Molecular Biology
Divisions: SCIENTIFIC PRODUCTION > Biochemistry, Genetics and Molecular Biology
Depositing User: Administrateur Eprints Administrateur Eprints
Last Modified: 31 Jan 2020 15:44
URI: http://eprints.umi.ac.ma/id/eprint/1542

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